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In vivo imaging of hematopoietic stem cell development in the zebrafish
Panpan Zhang, Feng Liu
《医学前沿(英文)》 2011年 第5卷 第3期 页码 239-247 doi: 10.1007/s11684-011-0123-0
关键词:
hematopoietic stem cell
hematopoiesis
Yongqing Zhou, Xin Wang, Wei Zhang, Bo Tang, Ping Li
《化学科学与工程前沿(英文)》 2022年 第16卷 第1期 页码 4-33 doi: 10.1007/s11705-021-2041-2
关键词: bioactive molecules fluorescent probes in living cells and in vivo review
SWIR Fluorescence Imaging In Vivo Monitoring and Evaluating Implanted M2 Macrophages in Skeletal Article
Mo Chen, Yuzhou Chen, Sijia Feng, Shixian Dong, Luyi Sun, Huizhu Li, Fuchun Chen, Nguyen Thi Kim Thanh, Yunxia Li, Shiyi Chen, You Wang, Jun Chen
《工程(英文)》 doi: 10.1016/j.eng.2023.05.010
Skeletal muscle has a robust regeneration ability that is impaired by severe injury, disease, and aging, resulting in a decline in skeletal muscle function. Therefore, improving skeletal muscle regeneration is a key challenge in treating skeletal muscle-related disorders. Owing to their significant role in tissue regeneration, implantation of M2 macrophages (M2Mø) has great potential for improving skeletal muscle regeneration. Here, we present a short-wave infrared (SWIR) fluorescence imaging technique to obtain more in vivo information for an in-depth evaluation of the skeletal muscle regeneration effect after M2Mø transplantation. SWIR fluorescence imaging was employed to track implanted M2Mø in the injured skeletal muscle of mouse models. It is found that the implanted M2Mø accumulated at the injury site for two weeks. Then, SWIR fluorescence imaging of blood vessels showed that M2Mø implantation could improve the relative perfusion ratio on day 5 (1.09 ± 0.09 vs 0.85 ± 0.05; p = 0.01) and day 9 (1.38 ± 0.16 vs 0.95 ± 0.03; p = 0.01) post-injury, as well as augment the degree of skeletal muscle regeneration on day 13 post-injury. Finally, multiple linear regression analyses determined that post-injury time and relative perfusion ratio could be used as predictive indicators to evaluate skeletal muscle regeneration. These results provide more in vivo details about M2Mø in skeletal muscle regeneration and confirm that M2Mø could promote angiogenesis and improve the degree of skeletal muscle repair, which will guide the research and development of M2Mø implantation to improve skeletal muscle regeneration.
关键词: In vivo Short-wave infrared Skeletal muscle Macrophage Regeneration
Xiang LI, Yun LUO, Chengtao WANG, Wenguang ZHANG, Yuanchao LI
《机械工程前沿(英文)》 2012年 第7卷 第1期 页码 66-71 doi: 10.1007/s11465-012-0302-y
Electron beam melting process was used to fabricate porous Ti6Al4V implants. The porous structure and surface topography of the implants were characterized by scanning electron microscopy (SEM) and digital microscopy (DM). The results showed that the pore size was around 600 and the porosity approximated to 57%. There was about±50 μm of undulation on implants surfaces. Standard implants and a custom implant coupled with porous sections were designed and fabricated to validate the versatility of the electron beam melting (EBM) technique. After coated with bone-like apatite, samples with fully porous structures were implanted into cranial defects in rabbits to investigate the in vivo performance. The animals were sacrificed at 8 and 12 weeks after implantation. Bone ingrowth into porous structure was examined by histological analysis. The histological sections indicated that a large amount of new bone formation was observed in porous structure. The newly formed bone grew from the calvarial margins toward the center of the bone defect and was in close contact with implant surfaces. The results of the study showed that the EBM produced Ti6Al4V implants with well-controlled porous structure, rough surface topography and bone-like apatite layer are beneficial for bone ingrowth and apposition.
关键词: electron beam melting process implant porous structure bone ingrowth
Yan Shi, Fangjun Huo, Yongkang Yue, Caixia Yin
《化学科学与工程前沿(英文)》 2022年 第16卷 第1期 页码 64-71 doi: 10.1007/s11705-021-2048-8
关键词: heterocyclic compound hydrogen sulfide ratiometric mitochondrial targeted
Oral product input to the GI tract: GIS an oral product performance technology
Gordon L. Amidon, Yasuhiro Tsume
《化学科学与工程前沿(英文)》 2017年 第11卷 第4期 页码 516-520 doi: 10.1007/s11705-017-1658-7
关键词: GIS in vivo predictive dissolution ketoconazole BCS subclassification supersaturation
注射大鼠脑神经膜的非洲爪蟾卵母细胞——一种创新的体外方法研究拟除虫菊酯对天然态离子通道的影响 Review
John Clark, Steve Symington
《工程(英文)》 2020年 第6卷 第5期 页码 515-521 doi: 10.1016/j.eng.2019.10.017
将大鼠脑神经膜微移植到非洲爪蟾卵母细胞的质膜中,是一种利用标准电生理学方式研究其天然态通道和受体的体外方法。在本文中,我们发现注射了成年大鼠脑神经膜的卵母细胞会引发对河豚毒素敏感的膜去极化内向离子流,经除虫菊酯类杀虫剂氯菊酯和溴氰菊酯处理后,该电流呈浓度依赖性增加。根据我们最初的方案,随着时间的推移,卵母细胞的健康水平会降低,不同批次的不同蛙类卵母细胞间与神经膜的结合程度也不同,这限制了该试验在调控问题上的实用性。对分析程序、数据接受标准和分析方法的一系列更改可显著提高试验的精度,从而提高性能。这些变化促使这种体外方法成为一种毒理学相关分析方法,以研究拟除虫菊酯对取自幼年和成年大鼠脑中的神经膜片段的离子通道的毒性作用。
在临床批准的免疫抑制剂药物作用下胎猪肾脏在成熟猴体内的发育情况 Article
Tsuyoshi Takamura, Kenji Matsui, Naoto Matsumoto, Yatsumu Saito, Toshinari Fujimoto, Susumu Tajiri, Shuichiro Yamanaka, Kei Matsumoto, Akimitsu Kobayashi, Izumi Yamamoto, Hiroshi Sasaki, Haruyuki Hirayama, Hitomi Matsunari, Kazuaki Nakano, Hiroshi Nagashima, Akihiko Kiyoshi, Takao Kuroda, Makoto Inoue, Takeshi Miyawaki, Takashi Yokoo, Eiji Kobayashi
《工程(英文)》 2022年 第10卷 第3期 页码 65-73 doi: 10.1016/j.eng.2022.02.001
在从猪到非人灵长类动物的肾脏异种移植中,仅使用临床批准的免疫抑制药物来控制免疫反应是困难的。此外,据我们所知,目前还没有关于使用胎猪作为肾脏供体的报道。本研究旨在比较新生猪和胎猪肾脏之间的移植排斥程度,以遗传未修饰的猪作为供体,将食蟹猴作为受体。切除受体食蟹猴的左肾,然后将在同一部位进行了血管吻合的新生猪和胎猪的肾脏移植到食蟹猴左肾腹膜。仅使用美国食品药品监督管理局批准的药物进行免疫抑制。将胎猪肾脏移植到食蟹猴的网膜和腹主动脉旁区域。随时间取样(每个实验取样两次),对移植组织的移植成活率和发育进行病理学检查。几周后,观察到血管吻合的新生猪肾脏在移植后出现急性排斥反应。尽管对食蟹猴和流入胎猪肾脏的受体血管给予相同的免疫抑制方案,但胎猪的肾脏仍免于排斥。猪-猴肾脏异种移植中胎猪肾脏的免疫原性低于新生猪肾脏。
标题 作者 时间 类型 操作
molecule fluorescent probes for simultaneous imaging of two bioactive molecules in live cells and in vivo
Yongqing Zhou, Xin Wang, Wei Zhang, Bo Tang, Ping Li
期刊论文
SWIR Fluorescence Imaging In Vivo Monitoring and Evaluating Implanted M2 Macrophages in Skeletal
Mo Chen, Yuzhou Chen, Sijia Feng, Shixian Dong, Luyi Sun, Huizhu Li, Fuchun Chen, Nguyen Thi Kim Thanh, Yunxia Li, Shiyi Chen, You Wang, Jun Chen
期刊论文
Fabrication and in vivo evaluation of Ti6Al4V implants with controlled porous structure and complex shape
Xiang LI, Yun LUO, Chengtao WANG, Wenguang ZHANG, Yuanchao LI
期刊论文
Positive ion activated rapid addition and mitochondrial targeting ratiometric fluorescent probes for in vivo
Yan Shi, Fangjun Huo, Yongkang Yue, Caixia Yin
期刊论文
Oral product input to the GI tract: GIS an oral product performance technology
Gordon L. Amidon, Yasuhiro Tsume
期刊论文
在临床批准的免疫抑制剂药物作用下胎猪肾脏在成熟猴体内的发育情况
Tsuyoshi Takamura, Kenji Matsui, Naoto Matsumoto, Yatsumu Saito, Toshinari Fujimoto, Susumu Tajiri, Shuichiro Yamanaka, Kei Matsumoto, Akimitsu Kobayashi, Izumi Yamamoto, Hiroshi Sasaki, Haruyuki Hirayama, Hitomi Matsunari, Kazuaki Nakano, Hiroshi Nagashima, Akihiko Kiyoshi, Takao Kuroda, Makoto Inoue, Takeshi Miyawaki, Takashi Yokoo, Eiji Kobayashi
期刊论文